This technique is used as a diagnostic tool in medicines or pathology related to plants. It has proved to be a good quality control check in industries involving food processing. Elisa's test is conducted on the antigen and antibody concentrations.
The ELISA technique is used to measure hormone levels, protein levels, peptides, and antibodies in the collected samples. There are five types of ELISA tests that can be done depending on the situation:
Direct ELISA – This type of test is considered a simple test that saves a lot of time. If you want to know more about ELISA testing, then you can also check out the Boster Bio featured products.
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In this type of test, the plastic plate is removed and the antigen is adsorbed on it. An additional amount of other protein is then added to the mixture to block other binding sites.
Indirect ELISA – This type of test is conventional, but many who use it have confirmed its effectiveness. In this type of test, various antibodies can be used for early detection along with labeled secondary antibodies.
ELISA sandwich – In this type of test, the antigen is taken as a substance and coated with two antibody layers; H. Captures antibodies and antibodies for detection and then calculates the results. Two types of antibodies are used in this test – monoclonal and polyclonal.
Competitive ELISA – The binding process achieved by adding a test antigen to a number of additional antigens can be competitively referred to as competitive ELISA. This type of test is different from other types of ELISA tests. In this type of test, if the sample has a higher antigen concentration, it is likely that the signal will be weak.